Ethnobotany and in vitro conservation of Zataria multiflora Boiss

Document Type : Original Article

Authors

Department of Agriculture, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Evin, Tehran, Iran. Postal Code: 1983969411.

10.22091/ethc.2023.9699.1005

Abstract

In this study, ethnobotanical data and in vitro conservation of Zataria multiflora Boiss. were investigated. By visiting the plant natural habitats in Fars, Isfahan, and Hormozgan Provinces, it was found that Z. multiflora is locally known as ‘Avishan-e-Shirazi’, ‘Avishan-e-Barg Pahn’, and ‘Avesham’ and used to treat human and animal diseases. Zataria multiflora habitats are being severely destroyed due to the plant materials over-collection from nature for many medicinal in the recent years. So, conservation of this valuable species is necessary. In this study, the indirect plant micropropagation was studied. Induced calli from the seedling were transferred to the Gamborg (B5) medium containing 6-benzyl amino purine (BAP) (0.1, 1.5 and 0.2 mg/l) with indole-3-butyric acid (IBA) (0.1 and 0.5 mg/l) and 2,4-dichlorophenoxyacetic acid (0.1 and 0.5 mg/l). The highest shoot-forming capacity (13.8) was obtained in the medium supplemented with 1.5 mg/l BAP and 0.1 mg/l IBA. The maximum root-forming capacity (6.8) was found in the medium supplemented with 1.0 mg/l IBA. The rooted plantlets were successfully acclimatized (75%). The results can be used to protect germplasm, supply seedlings, and restore the plant habitats.

Keywords

Main Subjects

References

Abd El-Motaleb, M., Abd El-Hameid, A. R., Helmy, W. A., Ewais, E. A., & Abdel-Hady, M. S. (2023). Establishment of callogenesis and plant regeneration protocols for endemic Origanum syriacum ssp. sinaicum. Journal of Crop Science and Biotechnology, 26(4), 525-536.
Aghamohammadi, A., Azadbakht, M., & Hosseinimehr, S. J. (2016). Quantification of thymol content in different extracts of Zataria multiflora by HPLC method. Pharmaceutical and Biomedical Research, 2(1), 8-13.
Alarcón, M. V., Salguero, J., & Lloret, P. G. (2019). Auxin modulated initiation of lateral roots is linked to pericycle cell length in maize. Frontiers in Plant Science, 10, 11.
Arvin, P., & Firouzeh, R. (2022). Ethnobotany of medicinal plants in Razo-Jargalan district in North Khorasan province. Iranian Journal of Medicinal and Aromatic Plants Research, 37(6), 873-907. (in Persian)
Bernard, F., Shaker, H., Hassanpoor, H., & Nejad Fallah, Z. (2007). Growth optimization of Zataria multiflora Boiss. tissue cultures and rosmarinic acid production improvement. Pakistan Journal of Biological Science, 10(19), 3395-3399.
Das, S., Sultana, K. W., & Chandra, I. (2023). In vitro propagation, phytochemistry and pharmacology of Basilicum polystachyon (L.) Moench (Lamiaceae): A short review. South African Journal of Botany, 155, 178-186.
Díaz-Pérez, J. C., Shackel, K. A., & Sutter, E. G. (1995). Effects of in vitro-formed roots and acclimatization on water status and gas exchange of tissue-cultured apple shoots. Journal of the American Society for Horticultural Science, 120(3), 435-440.
Echeverrigaray, S., Carrer, R. P., & Andrade, L. B. (2010). Micropropagation of Salvia guaranitica Benth. through axillary shoot proliferation. Brazilian Archives of Biology and Technology, 53, 883-888.
Gamborg, O. L., Miller, R., & Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50(1), 151-158.
Gepts, P. (2006). Plant genetic resources conservation and utilization: the accomplishments and future of a societal insurance policy. Crop Science, 46(5), 2278-2292.
Ghasemi, M., Mirjalili, M. H., & Hadian, J. (2014). Chemical profiles of the essential oil of wild and in vitro regenerated Zataria multiflora Boiss. (Lamiaceae). Bulgarian Chemical Communications, 46(2), 362-367.
Ghorani, V., Beigoli, S., Khazdair, M. R., & Boskabady, M. H. (2022). The effect of Zataria multiflora on respiratory allergic and immunologic disorders, experimental and clinical evidence: A comprehensive review. Phytotherapy Research, 36(3), 1135-1155.
Hashemi, S. A., Azadeh, S., Nouri, B. M., & Navai, R. A. (2017). Review of pharmacological effects of Zataria multiflora Boiss. (thyme of Shiraz). International Journal of Medical Research & Health Sciences, 6(8), 78-84.
Iranian Herbal Pharmacopoeia (2002). Ministry of Health and Medical Publications, Tehran (pp. 51-56). (in Persian)
Jamshidi-Kia, F., Lorigooini, Z., & Amini-Khoei, H. (2017). Medicinal plants: Past history and future perspective. Journal of Herbmed Pharmacology, 7(1),1-7.
Jan, M., Singh, S., & Maqbool, F. (2016). Micropropagation of some medicinally important plant species of Family Lamiaceae–A review. International Journal of BioSciences & Technology, 9(11), 64-73.
Leal, F., Taghouti, M., Nunes, F., Silva, A., Coelho, A.C., & Matos, M. 2017. Thymus plants: A review—Micropropagation, molecular and antifungal activity. Active Ingredients from Aromatic and Medicinal Plants, 1(7), 107-126.
Mahboubi, M. (2018). Management of acute cough by Zataria multiflora Boiss as an alternative treatment. Journal of Integrative Medicine, 16(1), 20-25.
Mahboubi, M. (2019). Therapeutic potential of Zataria multiflora boiss in treatment of irritable bowel syndrome (IBS). Journal of Dietary Supplements, 16(1), 119-128.
Minaiyan, M., Sajjadi, S. E., & Amini, K. (2018). Antiulcer effects of Zataria multiflora Boiss. on indomethacin-induced gastric ulcer in rats. Avicenna Journal of Phytomedicine, 8(5), 408.
Mozaffarian, V. (2013). Identification of medicinal and aromatic plants of Iran. Tehran: Farhang Moaser Publishers. (in Persian)
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
Narimani, R., Moghaddam, M., & Mojarab, S. (2016). Evaluation of the micropropagation of hairless catmint (Nepeta nuda L.), an endangered medicinal plant. Cell and Tissue Journal, 7(4), 387-397. (in Persian)
Naseri, M. K. G., Mazlomi, H., Goshaiesh, M., Vakilzadeh, G., & Heidari, A. (2006). Antispasmodic effect of Zataria multiflora Boiss. Leaf extract on the rat uterus. Iranian Journal of Pharmaceutical Research, 5(2),131-136.
Ogwu, M. C., Osawaru, M. E., & Ahana, C. M. (2014). Challenges in conserving and utilizing plant genetic resources (PGR). International Journal of Genetics and Molecular Biology, 6(2),16-22.
Omidbaigi, R. (2009). Production and processing of medicinal plants. Mashhad: Astan Quds Razavi Publishers. (in Persian)
Ozudogru, E. A., Kaya, E., Kirdok, E., & Issever-Ozturk, S. (2011). In vitro propagation from young and mature explants of thyme (Thymus vulgaris and T. longicaulis) resulting in genetically stable shoots. In Vitro Cellular & Developmental Biology-Plant, 47, 309-320.
Rechinger, Kh. (1982). Flora Iranica. Graz-Austria: Akademic Druck-u Verlagsanstalt, 150, 292-313.
Safa, O., Soltanipoor, M. A., Rastegar, S., Kazemi, M., Dehkordi, K. N., & Ghannadi, A. (2013). An ethnobotanical survey on hormozgan province, Iran. Avicenna Journal of Phytomedicine, 3(1), 64.
Saha, S., Dey, T., & Ghosh, P. (2010). Micropropagation of Ocimum kilimandscharicum Guerke (Labiatae). Acta Biologica Cracoviensia s. Botanica, 52(2), 50-58.
Sajed, H., Sahebkar, A., & Iranshahi, M. (2013). Zataria multiflora Boiss. (Shirazi thyme)-an ancient condiment with modern pharmaceutical uses. Journal of Ethnopharmacology, 145(3), 686-698.
Sefidkon, F. (2021). Selection of native and endemic medicinal plants of Iran for domestication. Iran Nature, 6(4), 149-149. (in Persian)
Tareen, R. B., Bibi, T., Khan, M. A., Ahmad, M., Zafar, M., & Hina, S. (2010). Indigenous knowledge of folk medicine by the women of Kalat and Khuzdar regions of Balochistan, Pakistan. Pakistan Journal of Botany, 42(3), 1465-1485.
 Tevfik, A. S., & Yegorova, N. A. (2020). Clonal micropropagation of Thymus vulgaris L. In E3S Web of Conferences (Vol. 224, p. 04001). EDP Sciences.

Files

Share

How to cite

Statistics