The effect of different concentrations of linoleic acid on physiological responses and production of bioactive compounds in Spirulina platensis

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qaemshahr Branch, Qaemshahr, Iran

2 Department of Biology, Faculty of Basic Science, Islamic Azad University, Sari Branch, Sari, Iran.

3 Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qaemshahr Branch, Qaemshahr, Iran.

10.22091/ethc.2025.11770.1043

Abstract

Objective: Spirulina platensis is an important source of bioactive compounds, especially plant pigments, phenolic compounds, and antioxidants. In this study, the effect of linoleic acid (30 and 60 μM) on redox and biochemical changes of Spirulina platensis was studied during 3 and 7 days.
Methods: In the present study, the content of superoxide anion, the activity of catalase and peroxidase enzymes, as well as the content of photosynthetic pigments, proline, ascorbate, phenol, and total flavonoids were calculated via spectrophotometer.
Results: The dry weight of algae treated with different concentrations of linoleic acid increased after 3 days. Also, the superoxide anion content increased significantly at a concentration of 30 μM linoleic acid after 7 days. Also, linoleic acid treatment reduced the activity of catalase and peroxidase enzymes. Also, this treatment at a concentration of 60 μM increased the content of total chlorophyll, chlorophyll a, chlorophyll b, and carotenoids. The content of proline and ascorbic acid decreased significantly in response to concentrations of 30 and 60 μM linoleic acid. A significant increase in total phenols and flavonoid content was observed in response to linoleic acid, with the highest content of total phenols and flavonoids observed at a concentration of 60 μM after 7 days.
Conclusion: As a result, it can be suggested that linoleic acid can alter biochemical and metabolic responses in Spirulina platensis by changing the content of superoxide anion as a signaling molecule.

Keywords

Main Subjects

References

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