Investigation of the variation in quantitative characteristics of essential oil of Ziziphora tenuior at different phenological stages

Document Type : Original Article

Authors

1 Department of Range and Watershed Management, Arak Branch, Islamic Azad University, Arak, Iran.

2 Department of Agriculture, Kerman Branch, Islamic Azad University, Kerman, Iran.

10.22091/ethc.2025.12691.1054

Abstract

Objective: The medicinal plant Ziziphora, widely favored in traditional Iranian medicine, is predominantly found in the southern and central parts of Iran. This study was conducted in the Baft region of Kerman Province to evaluate the effect of diffetent phenological stages on the essential oil content of Ziziphora tenuior.
Methods: This study employed an independent t-test design. The phenological stage was considered as the experimental factor at two levels: pre-flowering and flowering. Essential oil was extracted from plant samples using a Clevenger apparatus, and after dehydration, the obtained data were statistically analyzed.
Results: The results indicated thatharvest time had a significant effect on essential oil content. Samples harvested before flowering contained significantly lower amounts of essential oil compared to those harvested at the flowering stage. The essential oil content increased by more than twofold from the pre-flowering stage to the flowering stage, rising from 0.43% to 1.00%.
Conclusion: The findingsdemonstrated that as plant age increased and the phenological stage progressed from pre-flowering to flowering, the essential oil content increased significantly. This increase is likely associated with physiological changes in the plant and the enhanced production of volatile compounds during the flowering stage

Keywords

Main Subjects


Adams, R. P. (2017). Identification of essential oil components by gas chromatography/mass spectrometry (5th Ed.). Texensis Publishing. 
Amin, G., Sourmaghi, M. S., Azizzadeh, M., Yassa, N., & Asgari, T. (2008). Seasonal variation of the essential oil composition of cultivated yarrow in Tehran-Iran. Journal of Essential Oil-Bearing Plants, 11(6), 628–633. https://doi.org/10.1080/0972060X.2008.10643678
Amiri, F., Gholipouri, A., Kheirkhah, M., & Mirjalili, M. H. (2019). Study on ethnobotany and the effect of ecological factor on the yield of essential oil of Ziziphora clinopodioides Lam. (case study: Yazd Province). Journal of Medicinal Plants and By-Products, 8(2), 189–199. https://doi.org/10.22092/jmpb.2019.120497
Asgharipour, M. R., Abjahan, A. A., & Dahmarde, M. (2016). Variability in Ziziphora clinopodioides subsp. bungeana (Juz.) based on morphological traits and essential oils profile. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 44(1), 189–194. https://doi.org/10.15835/nbha44110118
Bączek, K., Kosakowska, O., Przybył, J. L., & Węglarz, Z. (2016). Accumulation of phenolic compounds in the purple betony herb (Stachys officinalis L.) originated from cultivation. Herba Polonica, 62(2), 7–16. https://doi.org/10.1515/hepo-2016-0007
Bakhtiar, A., Khaghani, S., Ghasemi Pirbalouti, A., Gomarian, M., & Chavoshi, S. (2021). Essential oil variation among different populations of Ziziphora tenuior L. cultivated at semiarid climate. Journal of Essential Oil Research, 33(4), 385–393. https://doi.org/10.1080/10412905.2021.1909666
Boostani, H. R., & Mahmoodi, A. (2024). Mineral content of some famous medicinal plants grown in Darab region, Southern Iran. Journal of Plant Process and Function, 12(58), 61–66. http://dx.doi.org/DOI:%2010.22034/12.58.61
De Hoffmann, E., & Stroobant, V. (2007). Mass spectrometry: Principles and applications. John Wiley & Sons. 
Dirmenci, T., Haloob, A., Celep, F., & Ghazanfar, S. A. (2023). Contributions to Ziziphora clinopodioides (Lamiaceae) in Turkey and Iraq. Phytotaxa, 592(3), 279–293. https://doi.org/10.11646/phytotaxa.592.3.6
Field, A. (2024). Discovering statistics using IBM SPSS statistics. Sage Publications Limited. 
Greer, B., Chevallier, O., Quinn, B., Botana, L. M., & Elliott, C. T. (2021). Redefining dilute and shoot: The evolution of the technique and its application in the analysis of foods and biological matrices by liquid chromatography mass spectrometry. TrAC Trends in Analytical Chemistry, 141, 116284. https://doi.org/10.1016/j.trac.2021.116284
Hatami, A. (2024). Phytochemical characterisation of dichloromethane and methanolic extracts of the Ziziphora tenuior leaves and evaluation of their antioxidant and antibacterial activities. Natural Product Research, 1–8. https://doi.org/10.1080/14786419.2024.2386127
Hazrati, S., Govahi, M., Sedaghat, M., & Kashkooli, A. B. (2020). A comparative study of essential oil profile, antibacterial and antioxidant activities of two cultivated Ziziphora species (Z. clinopodioides and Z. tenuior). Industrial Crops and Products, 157, 112942. https://doi.org/10.1016/j.indcrop.2020.112942
Hiremath, C., Yadav, M. K., Swamy Gowda, M. R., Kumar, A. N., & Srinivas, K. V. N. (2020). Influence of growth stage on essential oil content and major chemical constituents of Artemisia pallens Bess. Trends in Phytochemical Research, 4(2), 85–92.  https://dorl.net/dor/20.1001.1.25883623.2020.4.2.5.9
Kant, R., & Kumar, A. (2022). Review on essential oil extraction from aromatic and medicinal plants: Techniques, performance and economic analysis. Sustainable Chemistry and Pharmacy, 30, 100829. https://doi.org/10.1016/j.scp.2022.100829
Mehalaine, S., & Chenchouni, H. (2021). Quantifying how climatic factors influence essential oil yield in wild-growing plants. Arabian Journal of Geosciences, 14(13), 1257. https://doi.org/10.1007/s12517-021-07582-6
Mohammadhosseini, M. (2017). The ethnobotanical, phytochemical and pharmacological properties and medicinal applications of essential oils and extracts of different Ziziphora species. Industrial Crops and Products, 105, 164–192. https://doi.org/10.1016/j.indcrop.2017.05.009
Najafian, S. (2023). Phytochemical properties, volatile components, and herbage yield from leaves of Kakuti. Journal of Medicinal Plants and By-Products, 13(1), 1–9.  https://doi.org/10.22092/jmpb.2022.358659.1474
Nazari, M., Ghasemi-Soloklui, A. A., Kordrostami, M., & Latef, A. A. H. A. (2023). Deciphering the response of medicinal plants to abiotic stressors: A focus on drought and salinity. Plant Stress, 100255. https://doi.org/10.1016/j.stress.2023.100255
Nourani, M. (2014). The great encyclopedia of Islamic medicine. Mobin Andisheh. (in Persian)
Panahy, J., Teymurzadeh, A., & Panahy, A. (2011, November). An ethnobotanical survey in southern slopes of Sabalan Mountain. In International Symposium on Medicinal and Aromatic Plants IMAPS2010 and History of Mayan Ethnopharmacology IMAPS2011 (Vol. 964, pp. 13–21). 
Pant, P., Pandey, S., & Dall’Acqua, S. (2021). The influence of environmental conditions on secondary metabolites in medicinal plants: A literature review. Chemistry & Biodiversity, 18(11), e2100345. https://doi.org/10.1002/cbdv.202100345
Pourmirzaie, A. (2021). The Khabr National Park and Rouchun Wildlife as the only national park in the southeast of the country. Iran Nature, 6(5), 91–100. https://doi.org/10.22092/irn.2021.355830.1405 (in Persian)
Sadat Sarvghad, A., & Solaimani Doostabad, A. (2023). Investigation of the chemical compounds of the medicinal plant Ziziphora clinopodioides Lam and its role in the treatment of diseases. Journal of Agriculture and Sustainable Environment Research, 3(4), 27-40. (in Persian)
Selvi, S., Satil, F., Martin, E., Çelenk, S., & Dirmenci, T. (2015). Some evidence for infrageneric classification in Ziziphora L. (Lamiaceae: Mentheae). Plant Biosystems—An International Journal Dealing with All Aspects of Plant Biology, 149(2), 415–423. https://doi.org/10.1080/11263504.2013.853701
Tabaripour, R., Sheidai, M., Talebi, S. M., & Noormohammadi, Z. (2018). Genetic divergence and speciation within Ziziphora capitata (Lamiaceae): Molecular and micromorphological evidences. Biodiversitas Journal of Biological Diversity, 19(2), 697–705. https://doi.org/10.13057/biodiv/d190250
Taheri, A., Ganjeali, A., Arefi-Oskouie, A., Çirak, C., & Cheniany, M. (2023). The variability of phenolic constituents and antioxidant properties among wild populations of Ziziphora clinopodioides Lam. Physiology and Molecular Biology of Plants, 29(2), 221–237. https://doi.org/10.1007/s12298-023-01283-y
Uritu, C. M., Mihai, C. T., Stanciu, G. D., Dodi, G., Alexa-Stratulat, T., Luca, A., Leon-Constantin, M. M., Stefanescu, R., Bild, V., Melnic, S., & Tamba, B. I. (2018). Medicinal plants of the family Lamiaceae in pain therapy: A review. Pain Research and Management, 2018, 7801543. https://doi.org/10.1155/2018/7801543
Watson, J. T., & Sparkman, O. D. (2007). Introduction to mass spectrometry: Instrumentation, applications, and strategies for data interpretation. John Wiley & Sons. 
Zhaparkulova, K., Karaubayeva, A., Sakipova, Z., Biernasiuk, A., Gaweł-Bęben, K., Laskowski, T., Kusniyeva, A., Omargali, A., Bekezhanova, T., Ibragimova, L., & Ibadullayeva, G. (2022). Multidirectional characterization of phytochemical profile and health-promoting effects of Ziziphora bungeana Juz. Extracts. Molecules, 27(24), 8994. https://doi.org/10.3390/molecules27248994