Cultivation and Domestication of Thymbra spicata L., a Medicinal Plant

Document Type : Original Article

Authors

1 Lorestan Agricultural and Natural Resources Research and Education Center, AREEO, Khorramabad, Iran

2 IInstitude of forest and rangelands, AREEO Tehran, Iran.

10.22091/ethc.2026.15770.1080

Abstract

Objective: Thymbra spicata L. is a perennial evergreen shrub of the Lamiaceae family, naturally distributed in western Iran and widely recognized for its medicinal properties. Traditionally, it has been used to treat sore throat, oral ulcers, stomachache, headache, and toothache. Due to increasing demand and pressure on natural populations, this study aimed to evaluate the feasibility of its domestication and cultivation by investigating the effects of propagation methods and irrigation regimes on biomass production and essential oil yield.
Methods: A factorial experiment based on a randomized complete block design with three replications was conducted under field conditions. Treatments consisted of two irrigation regimes (rainfed and irrigation at 10-day intervals) and three propagation methods (seed, stem cuttings, and plant division). Fresh and dry biomass production, essential oil yield, and phenological characteristics were evaluated.
Results: The highest fresh biomass (18,266.67 kg ha⁻¹), dry biomass (6,026.67 kg ha⁻¹), and essential oil yield (254.12 kg ha⁻¹) were obtained from plants propagated by stem cuttings under irrigated conditions. These values were significantly higher than those of the other propagation methods.
Conclusion: The results demonstrated that irrigation significantly improved plant height, lateral branching, and fresh and dry biomass production. Among the propagation methods, stem cuttings ensured faster establishment, greater aboveground growth, and superior biomass yield compared with plant division. Irrigation enhanced fresh biomass by about twofold in divided plants and 1.7-fold in stem-cutting-propagated plants. Overall, the combination of stem cutting propagation and regular irrigation proved to be the most efficient strategy for successful domestication, maximizing biomass production, and achieving economically sustainable cultivation of Thymbra spicata L. under cultivated conditions

Keywords

Main Subjects


Akin, M., Oğuz, D., & Saraçoğlu, H. (2010). Antibacterial activity of essential oil from Thymbra spicata var. spicata L. and Teucrium polium (Stapf Brig.). Interventions, 8(9), 53–58.
Anderson, W., Johansen, C., & Siddique, K. H. M. (2016). Addressing the yield gap in rainfed crops: A review. Agronomy for Sustainable Development, 36, 1–13.
Bahreininejad, B., Lebaschy, M. H., Sefidkon, F., & Jaberalansar, Z. (2021). Effects of planting bed on vegetative characteristics and yield of Satureja sahendica and Satureja spicigera species under rainfed conditions. Iranian Journal of Field Crops Research, 19(4), 407–419. (in Persian)
Baydar, H., Sagdic, O., Ozkan, G., & Karadogan, T. (2004). Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control, 15(3), 169–172.
Farland, E. J., Skaugen, T. E., Benestad, R. E., Hanssen-Bauer, I., & Tveito, O. E. (2004). Variations in thermal growing, heating, and freezing indices in the Nordic Arctic, 1900–2050. Arctic, Antarctic, and Alpine Research, 36(3), 347–356.
Fazeli Kakhki, S. F., Jalini, M., Rezvani, H., & Goladani, M. (2020). The effect of periodic irrigation on morphological characteristics, yield components and seed oil of evening primrose under field conditions. Water and Sustainable Development, 7(3), 73–82. (in Persian)
Garivani, G., Sharifi Ashoorabadi, E., Safari, S., & Mirza, M. (2014). Assessment of domestication and harvesting time on growth and concentrations of active ingredients of two thyme (Thymus L.) species in North Khorasan Province of Iran. Iranian Journal of Medicinal and Aromatic Plants Research, 30(3), 445–452. (in Persian)
Grigorieva, E. (2020). Evaluating the sensitivity of growing degree days as an agro-climatic indicator of climate change impact: A case study of the Russian Far East. Atmosphere, 11(4), Article 404.
Grigorieva, E. A., Matzarakis, A., & de Freitas, C. R. (2010). Analysis of the growing degree days as a climate impact indicator in a region with extreme annual air temperature amplitude. Climate Research, 42(2), 143–154.
Haroun, M. F., & Al-Kayali, R. S. (2016). Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug-resistant Staphylococcus aureus and Klebsiella pneumoniae strains. Iranian Journal of Basic Medical Sciences, 19(11), 1193–1200.
Jalili, A., & Jamzad, Z. (1999). Red data book of Iran: A preliminary survey of endemic, rare and endangered plant species in Iran (Publication No. 215). Research Institute of Forests and Rangelands.
Jamzad, Z. (2012). Flora of Iran (Vol. 76: Lamiaceae). Research Institute of Forests and Rangelands. (in Persian)
Kilic, T. (2006). Analysis of essential oil composition of Thymbra spicata var. spicata: Antifungal, antibacterial and antimycobacterial activities. Zeitschrift für Naturforschung C, 61(5–6), 324–328.
Kizil, S., & Toncer, O. (2005). Effect of row spacing on seed yield, yield components, fatty oil and essential oil of Nigella sativa L. Crop Research-Hisar, 30(1), 107.
Kizil, S., Toncer, O., Diraz, E., & Karaman, S. (2015). Variation of agronomical characteristics and essential oil components of zahter (Thymbra spicata L. var. spicata) populations under semi-arid climatic conditions. Field Crops Central Research Institute Journal, 20(2), 242–251.
MalekMaleki, F., Abasi, N., Sharifi Ashoorabadi, E., Barari, M., & Zare, M. (2018). Effects of row distance on yield of two ecotypes of Thymbra spicata L. under field growing conditions. Iranian Journal of Medicinal and Aromatic Plants Research, 34(1), 62–76. (in Persian)
Marković, T., Chatzopoulou, P., Šiljegović, J., Nikolić, M., Glamočlija, J., Ćirić, A., & Soković, M. (2011). Chemical analysis and antimicrobial activities of the essential oils of Satureja thymbra L. and Thymbra spicata L. and their main components. Archives of Biological Sciences, 63(2), 457–464.
Rao, C. S., Lal, R., Prasad, J. V. N. S., Gopinath, K. A., Singh, R., Jakkula, V. S., Sahrawat, K. L., Venkateswarlu, B., Sikka, A., & Virmani, S. M. (2015). Potential and challenges of rainfed farming in India. Advances in Agronomy, 133, 113–181.
Sarac, N., Ugur, A., & Duru, M. E. (2009). Antimicrobial activity and chemical composition of the essential oils of Thymbra spicata var. intricata. International Journal of Green Pharmacy, 3(1), 24–28.
Schippmann, U., Leaman, D., & Cunningham, A. B. (2006). A comparison of cultivation and wild collection of medicinal and aromatic plants under sustainability aspects. In R. J. Bogers, L. E. Craker, & D. Lange (Eds.), Medicinal and aromatic plants (pp. 75–95). Springer.
Sharifi Ashoorabadi, E., Lebaschy, M. H., Naderi, B., & Allahverdi Mamaghani, B. (2009). Effect of water deficit on yield and essential oil of Achillea millefolium L. Environmental Sciences, 7(1), 193–204. (in Persian)
Sharifi Ashoorabadi, E., Jamzad, Z., Lebaschy, M. H., Akbarinia, A., Safaei, L., Larti, M., Habibi, R., Garivani, G. M., Safari, S., Samady Asl, V., & Mackizadeh Tafti, M. (2018). Applying thermal index to predict the phenological stages of Thymus growth in natural habitats. Iran Nature, 2(6), 34–44. (in Persian)
Toncer, O., Karaman, S., Diraz, E., Sogut, T., & Kizil, S. (2016). Diurnal variation effects on essential oils of wild thyme (Thymbra spicata var. spicata L.) under cultivation conditions. Journal of Essential Oil-Bearing Plants, 19(8), 2037–2048.
Zarekia, S., Ehsani, A., Zare, N., & Mirhaji, T. (2011). Phenological study of Astragalus chaborasicus, Poa sinaica, and Stipa hohenackeriana using growing degree days (GDD) in the Khoshkrood region of Saveh. Iranian Journal of Range and Desert Research, 18(3), 474–485. (in Persian)